|
KMID : 0545120170270122156
|
|
Journal of Microbiology and Biotechnology
2017 Volume.27 No. 12 p.2156 ~ p.2164
|
|
|
Soluble Prokaryotic Expression and Purification of Bioactive Tumor Necrosis Factor-Related Apoptosis-Inducing Ligand
|
|
Do Bich Hang
Nguyen Minh Tan
Song Jung-A
Park Sang-Su
Yoo Ji-Won
Jang Jae-Pyeong
Lee Sun-Ju
So Seoung-Jun
Yoon Ye-Jin
Kim In-Ki
Lee Kyung-Jin
Jang Yeon-Jin
Choe Han
|
|
|
Abstract
|
|
|
|
Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) is considered as an antitumor agent owing to its ability to induce apoptosis of cancer cells without imparting toxicity toward most normal cells. TRAIL is produced in poor yield because of its insoluble expression in the cytoplasm of E. coli. In this study, we achieved soluble expression of TRAIL by fusing maltose-binding protein (MBP), b’a’ domain of protein disulfide isomerase (PDIb’a’), or protein disulfide isomerase at the N-terminus of TRAIL. The TRAIL was purified using subsequent immobilized metal affinity chromatography and amylose-binding chromatography, with the tag removal using tobacco etch virus protease. Approximately 4.5 mg of pure TRAIL was produced from 125 ml flask culture with a purification yield of 71.6%. The endotoxin level of the final product was 0.4 EU/μg, as measured by the Limulus amebocyte lysate endotoxin assay. The purified TRAIL was validated and shown to cause apoptosis of HeLa cells with an EC50 and Hill coefficient of 0.6 ± 0.03 nM and 2.41 ± 0.15, respectively. The high level of apoptosis in HeLa cells following administration of purified TRAIL indicates the significance and novelty of this method for producing high-grade and high-yield TRAIL.
|
|
|
KEYWORD
|
|
|
Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL), expression, purification, E. coli, cytotoxic activity, HeLa cells
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
  
|